Enhancing the Innate Immune System with Brolico®


Introduction to Brolico® and its Health Benefits

During a collaborative research project conducted by Imagine Global Care Co., Ltd., University of Tokyo and Genome Pharmaceuticals Institute, research scientists discovered a new phytonutrient found in and extracted from the vegetable broccoli.  The new phytonutrient is called brolico.

Brolico cannot be absorbed by the body by eating broccoli, since it is expelled by the body without being absorbed.  Because of this limitation, scientists developed a patented extraction method which resulted in a supplemental form of brolico called Brolico® which can be absorbed by the body.  The Brolico® extraction method is patented under US Patent number 8,313,779, which was recorded in November 2012.

In various studies of the Brolico® supplement, researchers discovered that the brolico nutrient is able to produce high levels of cellular activity within crucial white blood cells, in effect, raising innate or natural immunity.  In fact, the Brolico® supplement produced record-breaking levels of activation among crucial white blood cells such as:

  • natural killer cells (NK cells)
  • neutrophils

The effects of Brolico® are very promising on immune system function:

  • Increases NK cell activity approximately 60 times more than fucoidan*
  • Helps and supports the defense mechanism of your immune system*
  • Increases natural killer cell activity*
  • Increase and elevates neutrophils*
  • Decreases the pro-inflammatory cytokine Interleukin 6 (IL-6)*
  • Promoted elimination of infected or harmful cells from the body*

The Human Immune System and Innate Immune System

The human immune system is a defense system that is composed of many biological structures and processes with the purpose of protecting against disease.  The immune system is classified into two sub-systems:

  • Innate immune system
  • Adaptive immune system

Image result for Innate immune system

Source:  StudyBlue

The innate immune system, also called the natural or native immune system, is based on the genetic constitution of the person, without any external stimulation or previous infection.  It is considered the first-line of defense of the body’s biological system from various pathogens.  The innate immune system does not provide long-lasting immunity to the host, but provides immediate defense against infection.

Innate immunity is divided into two types:

  • Non-Specific innate immunity: A degree of natural resistance to all infections in general
  • Specific innate immunity: This is a natural resistance to a particular kind of germ only. 

The major functions of the innate immune system include:

  • Recruiting immune cells to sites of infection, through the production of chemical factors, including specialized chemical mediators, called cytokines
  • Activation of the complement cascade to identify bacteria, activate cells, and promote clearance of antibody complexes or dead cells
  • Identification and removal of foreign substances present in organs, tissues, blood and lymph, by specialized white blood cells
  • Activation of the adaptive immune system through a process known as antigen presentation
  • Acting as a physical and chemical barrier to infectious agents

The adaptive immune system, is composed of highly specialized, systemic cells and processes that eliminate pathogens or prevent their growth. Adaptive immunity creates immunological memory after an initial response to a specific pathogen, and leads to an enhanced response to subsequent encounters with that pathogen.

Components of the immune system
Innate immune system Adaptive immune system
Response is non-specific Pathogen and antigen specific response
Exposure leads to immediate maximal response Lag time between exposure and maximal response
Cell mediated & 

humoral components

Cell mediated and 

humoral components

No immunological memory Exposure leads to immunological memory
Found in nearly all forms of life Found only in jawed vertebrates

Source:  Wikipedia:  Immune System

Two very important components of the innate immune system are neutrophil and natural killer cells (NK cells).

Neutrophil are the most abundant type of granulocytes and the most abundant (40% to 75%) type of white blood cells. During the beginning (acute) phase of inflammation, neutrophils are one of the first-responders of inflammatory cells to migrate towards the site of inflammation.

Natural killer cells or NK cells are a type of cytotoxic lymphocyte created in the bone marrow of the lymphatic system and provide rapid responses to viral-infected cells, acting at around 3 days after infection, and respond to tumor formation.  Natural killer (NK) cells, known to play a major role in the innate immune system, constantly patrol the body and destroy cancer cells and virus-infected cells.

Increasing neutrophil and NK cell activity is important to enhancing the innate immune system and defending the body by pathogens.  In fact, NK cells are the body’s first line of defense against the daily onslaught of pathogens.  Therefore optimizing NK cells provides greater protection and defense against foreign invaders.

T-cells and macrophages secrete Interleukin 6 (IL-6) to stimulate an immune response.  IL-6 acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine.  IL-6 stimulates the inflammatory and auto-immune processes in many diseases.  An elevated and excessive secretion of IL-6 may increase the risk of many diseases, especially atherosclerosis.

Studies on the Effectiveness of Brolico® Enhancing the Innate Immune System

A 2011 study entitled Comparison Study to Determine Immunity Activation Effectiveness Produced by Functional Food Extracts with Proposed Immunity Stimulation Effects, revealed a comparison study of various well known natural immunity activating extracts using a new active assay (silkworm muscle contraction), which was originally developed by faculty at Tokyo University Department of Pharmaceutical Sciences. 

Scientists discovered that when injecting a silkworm with a component that activates natural immunity, such as a bacteria or fungus, the muscles of the silkworm gradually shrink.  This is due to the fact that when the activated component binds with the immune cell of the silkworm, active oxygen is released, and a specific protein is activated, which causes the muscle to contract.   This muscle contraction can be measured by the degree of contraction in order to determine the precise level of specific activity (immunity activation) produced by the given component.

The researchers of this study used 10 extracts that are attributed to effect immunity stimulation for comparison against Brolico®.  These 10 extracts were:

  • açaí (berry fruit from Euterpe oleracea)
  • bee propolis
  • beta-glucan (from cell walls of cereals, yeast and bacteria)
  • broccoli sprouts
  • DHA (omega-3 fatty acid)
  • EPA (omega-3 fatty acid)
  • fucoidan (fund in brown algae)
  • lentinan (from Shiitake mushroom)
  • sulforaphane (from broccoli)

The results of the test were quite astonishing and showed that the phytonutrient extract of brolico (Brolico®) had the highest level of specific activity measuring approximately 770 units per milligram.  The other 10 extracts measured far below Brolico® with lentinan measuring the second highest with 290 units per milligram.

The Table below shows the measurements of each extract’s performance (units of specific activity per milligram), as well as the comparison evaluation results for each and Brolico®:

Comparison of Brolico® and 10 Extracts

ExtractsSpecific Activity (units/mg)Performance Comnparison
Lentinan (from Shiitake mushroom)290Approx 2.6x more
Broccoli sprouts (crude extracts)1840x more
Beta-glucan (from cell walls of cereals, yeast and bacteria)14Approx 50x more
Fucoidan (fund in brown algae)12Approx 60x more
DHA (omega-3 fatty acid)1070x more
Sulforaphane (from broccoli)7100x more
EPA (omega-3 fatty acid)4190x more
Açaí (berry fruit from Euterpe oleracea)3.1Approx 240x more
Bee propolis (ethanol extracts, crude extracts)0.401000x more

The researchers concluded that:

“the results suggest brolico to be capable of producing extraordinarily high levels of natural immunity stimulation, much higher those produced by its counterparts. Thus, there appears a significant benefit to further research on a much broader scale, specifically in regards to human trials.”   1


Source:  brolico.com

A research study published in 2012 in the Journal Japanese Pharmacology & Therapeutics (Vol.40 no.6 2012) entitled Exploratory Trial Concerning Activation of Human Natural Immunity by Continued Consumption of a Broccoli Extract Processed Food Product investigated the activating effect on human immune cells and safety of continuously consumed Brolico®.

Researchers at the University of Tokyo Graduate School of Pharmaceutical Sciences have found that muscles of silkworm larvae contract by the action of the innate immune system.  Researchers at Imagine Global Care Co., Ltd. in collaboration with the University of Tokyo Graduate School of Pharmaceutical Sciences sought out immunostimulatory substances using muscle contraction of silkworms as an indicator.  What they found was that Brolico® was found to have the potential for displaying an immunostimulatory effect that may exceed that of beta-glucan.

Test subjects were tested for three endpoints before and after consuming Brolico®.  These three endpoints included:

  • Neutrophil phagocytic capacity 
  • NK cell activity 
  • IL-6 levels

Changes in natural immunity-mediating cells NK cell activity and neutrophil phagocytic capacity tended to increase after consumption of the test food (p < 0.1 for both) (See Table 1). Levels of the pro-inflammatory cytokine, IL-6 actually decrease after consumption.  No significant changes in the hemogram levels were noted between the values before and after consumption.


Source:  Exploratory Trial Concerning Activation of Human Natural Immunity by Continued Consumption of a Broccoli Extract Processed Food Product

The researchers concluded their study with the promising statement:

“The observed trend toward increased NK cell activity and neutrophil phagocytic capacity after consumption of the test food suggests that brolico contributes to activation of cells involved in natural immunity.”   2

This study from 2012 suggests that the extracted broccoli constituent enhances innate immunity.

Informational References:

Brolico Research


Brolico® can be purchased at brolico.com

*The statements on this page have not been evaluated by the FDA. Products mentioned on this page are not intended to diagnose, treat, cure or prevent any disease. The information on this page is for educational purposes only, and does not constitute medical advice. For medical advice please contact a licensed healthcare professional.